Hey AML
The protein weights cross over. What each western-blot is measuring is protein weight in kilodaltans. They say okay. An HIV test... generally we will see these protein antibodies go positive. Lyme test, these proteins will go positive... e.g. 31, 34, 39, 41. Mono might also have different bands but includes 41 kDa. The proteins do cross react over between diseases if they have the same molecular weight but might not even be the same antibody protein. Some argue 41 is the first band to go positive in lyme disease but then others will argue it goes positive in epstein barr virus. So I think some bands do actually cross over.