Posted 3/2/2015 8:27 PM (GMT 0)
I just can't comprehend what this means. I finished some college last year in nursing but I'm still not sure. If any one can explain a little I would greatly appreciate it. My daughter has Downs and Lyme w/co infections.
B. henselae infection of endothelial progenitors
EPCs isolated from young euploid and DS individuals were infected after 3 days of culture with B. henselae at different MOI as described elsewhere [26] (Figure 2A). TEM examination confirmed that B. henselae is internalized by endothelial cells as bacterial aggregates within invasomes or as single bacteria by protrusions of the cells. Interestingly, the EPC number was dramatically impaired in both DS and euploid after bacteria internalization [Additional file 3: Supplemental Figure S1D]. In contrast, by confocal microscopy at high magnifications (x630), a more detrimental effect was observed in infected DS progenitors, showing some morphological major differences compared to euploid EPCs when the same non-lethal MOI of Bartonella was used (Figure 2A). Ultrastructural analysis revealed that infected DS progenitors have increased intracellular accumulation of bacteria, forming invasomes, compared to euploid cells infected at the same MOI of 100 (Figure 2B). Cytoplasmic protrusions of cell membranes were also observed in both samples following adherence of the bacteria to the host cells. Moreover, after infection at higher MOI (250), DS progenitors showed larger invasomes, also displaying, in some cases, invasome and cell membrane rupture with subsequent bacteria outflow (Figure 2B). In contrast, infected euploid cells showed significant lower number of invasomes. The number of infected DS cells was estimated to be significantly higher compared to euploid cells at both MOI used (Figure 2C). We did not use infection at MOI ≥500 of B. henselae since they were not compatible with DS progenitors' survival.