Western Blot, Manufactured Bb? How

https://www.lymedisease.org/wp-content/uploads/2014/08/Image13-link-pdf-brenner.pdf

I got this article from an earlier thread. What I don't understand from this article is how the lab obtains the borrelia burgdoferi Bb cells to create the proteins to then expose to the patient's blood. The article states the following:

"In a Western blot, the testing laboratory looks for antibodies directed against a
wide range of Bb proteins. This is done by first disrupting Bb cells with an electrical current
and then “blotting” the separated proteins onto a paper or nylon sheet."

From reading about the western blot on an HIV site, it sounds like the Bb cells are manufactured. In a Lyme Western Blot, are the Bb cells, which are then disrupted, manufactured cells? Is some lab somewhere growing Lyme borrelia in test tubes?

PLEASE NOTE: THIS IS NOT A QUESTION about INTERPRETING TEST RESULTS OR POSITIVE LYME BANDS. I'M JUST TRYING TO UNDERSTAND HOW THE Bb CELLS FOR THE TEST ARE OBTAINED.

Many thanks for your help, as always! Aylett

Thanks Traveler. That makes sense. What doesn't make sense is the FDA prosecuting people for looking at and identifying Lyme bacteria in blood under a microscope, when labs are growing Lyme bacteria elsewhere.

What wrong with someone saying, "I got bit by a tick, would you look at my blood?"
And someone else looking and saying, "I see corkscrew bacteria with flagella. That's most likely Lyme."

I guess I'm just shocked to find out that people are not allowed to identify it, when companies are growing it for profit. Thanks again, Aylett

Actually, with a DNA beacon, they can look at the bacteria and know that it's Lyme or not. They use a solution that only responds to the DNA of the Lyme bacteria and so only the Lyme bacteria will light up (blue I think?).

But, the CDC has a tight grip on "accepted" testing. That's why they keep trying to discredit the labs that are specialized in testing for Lyme - they only want their labs used, even though those are the labs that keep returning high numbers of false negatives. Igenex is one lab that has been attacked a lot over the years that it's been in business, so they have been very careful to be very open about being full accredited (www.igenex.com/why-igenex/licenses-accreditations/) - other Lyme specialty labs have had to go through a lot of the same things. Ridiculous things are being said about these labs (www.nytimes.com/2005/08/23/health/policy/unproved-lyme-disease-tests-prompt-warnings.html) and the CDC says that any test that doesn't follow Elisa/IFA testing is not valid either (what????) /www.cdc.gov/lyme/diagnosistesting/labtest/otherlab/index.html

Thanks, but I don't want to grow cultures.  I had a lady look at my blood with 5000x microscope.  A comrade of hers had been doing same.  She said that he advertised and the FDA swooped in on his office and confiscated his files and $6000 worth of equipment.  She is an ILADS member, yet won't allow me to give her name even to a doctor without her approval.  It's awful that this good hearted lady is operating in such fear of our government, when she is doing nothing wrong.  She found biofilm pieces, active spirochetes, and ones that were turning cell wall deficient.  I saw them when she put my blood on the glass plate under the microscope, because there was a camera that projected onto a computer screen.  After  2 years of "negative tests", it was comforting to find someone who actually directly helped me to see and know that I have lyme.  The fact that our government considers this nice lady a criminal is infuriating to me.  Especially, since companies are growing Lyme for profit. Traveler: thanks for the info.  How much does DNA ID cost in equipment.

Post Edited (Aylett) : 7/3/2017 4:09:45 AM (GMT-6)