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May have found a way to disable and treat Lyme neurotoxin

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Posted 10/17/2017 1:32 AM (GMT 0)
A researcher found that lyme bacteria secrete a neurotoxin he calls Bbtox1. It attacks adrenal cells and glial cells. "The glial cells surround neurons and provide support for and insulation between them. Glial cells are the most abundant cell types in the central nervous system."

interesting that so many lymies have adrenal issues and nerve issues.


http://www.actionlyme.org/Donta.htm

Somebody said...

A Novel Toxin (Bb Tox 1) of Borrelia burgdorferi

Mark J. Cartwright, Ph.D.*, Suzanne E. Martin, Ph.D. and Sam T. Donta, M.D.

The mechanisms responsible for many of the symptoms of Lyme disease remain to be delineated. Because many of the symptoms involve the nervous system,

we postulated that the Lyme spirochetes produce a toxin that interferes with normal neurophysiological function.

We have identified and cloned a gene of B. burgdorferi which encodes a protein that is a neurotoxin.

Initially, degenerate primers were designed to highly conserved regions within various toxin groups. These primers were used for amplification of DNA extracted from B. burgdorferi strain 2591 to identify genes that express proteins analogous to existing toxins. Degenerate primers designed to the highly conserved catalytic domains of diphtheria and pertussis toxins yielded an amplification product. The product was cloned, sequenced, and subsequently identified in The Institute of Genomic Research (TIGR) database as BB0755, a 37 kD protein of unknown function. The full length gene for BB0755 was cloned, expressed and purified using epitope tags in the pET30a expression system, and the resultant recombinant protein renamed Bbtox1. Using the synthetic target agmantine, Bbtox1 exhibited ADP-ribosyltransferase activity. No ADP-ribosyltransferase activity was detected using elongation factor 2 as the target.

In tissue culture, Bbtox1 affected the morphology (rounding) of Y1 mouse adrenal cells and C6 rat glial cells.

Bbtox1 induced cell death in both Y1 and C6 cells. C6 glial cells responded to Bbtox1 in a dose and time dependent manner. Brefeldin A, an inhibitor of the trans-golgi network, accelerated the onset of action of Bbtox1 an Y1 adrenal cells.

The effects of Bbtox1 are consistent with a mechanism of action similar to that of botulinum C2 and other cytoskeletal toxins.

Studies are underway to identify the cellular target of Bbtox1 and its role in Lyme Disease. In addition, a homologous gene in Treponema pallidum of undefined function is being analyzed to determine if it codes for a toxin similar to Bbtox1.


continued in next post
Posted 10/17/2017 1:44 AM (GMT 0)
the critical info here is this

"The effects of Bbtox1 are consistent with a mechanism of action similar to that of botulinum C2 and other cytoskeletal toxins."

so the lyme neuro toxin acts in the same way the botulism neurotoxin does.

Q: what is the mechanism of the botulism neurotoxin?

A: "Botulinum toxin (BTX) is a neurotoxic protein produced by the bacterium Clostridium botulinum and related species. It prevents the release of the neurotransmitter acetylcholine from axon endings at the neuromuscular junction and thus causes flaccid paralysis. Infection with the bacterium causes the disease botulism. "

so BTX prevents acetylcholine from being released, this causes nerves to shut down and all kinds of neuro muscular problems...much like neuro lyme patients complain of!

how is BTX treated?

with a specially designed antitoxin which is very very hard to get hold of.

so what can we do? how bout we look at substances that induce the release of acetylcholine

there are several one being DMSO

https://www.ncbi.nlm.nih.gov/pubmed/6704158

in that study they clearly show that DMSO blocked acetylcholinerase, the enzyme that breaks down acetylcholine, and stimulated the production of higher levels of acetylcholine

continued in next post
Posted 10/17/2017 2:06 AM (GMT 0)
so we see the lyme neurotoxin depresses acetylcholine levels, so to treat it we need a substance that does the opposite such as DMSO

another substance that increases acetylcholine levels is Huperzine A, available w/o a prescription. this study clearly shows that

https://www.ncbi.nlm.nih.gov/pubmed/16364207

Prescription drugs that increase acetylcholine are called Cholinesterase inhibitors and include Pyridostigmine. These may very well be helpful to neurolyme patients, but i have never heard of a LLMD using them

https://en.wikipedia.org/wiki/Acetylcholinesterase_inhibitor

but just increasing acetylcholine production is not enough, you also much give your body the necessary building blocks to create acetylcholine. Luckily that is easy to come by - choline supplements.

To Sum UP

the lyme bacteria secrete a neurotoxin called Bbtox1 that disables acetylcholine production.

To treat this you need to take something to stimulate acetylcholine production + choline supplement.

so treatment would be something like

DMSO + choline
Huperzine A (+EGCG)+ choline
Pyridostigmine. + choline

Post Edited (Lapis_29) : 10/17/2017 5:42:37 PM (GMT-6)

Posted 10/17/2017 2:11 AM (GMT 0)
here is my thread on DMSO, which is an amazing substance

https://www.healingwell.com/community/default.aspx?f=30&m=3914807
Posted 10/17/2017 3:04 AM (GMT 0)
Makes sense...Good info
Posted 10/17/2017 7:38 AM (GMT 0)
A great topic, but I wish it were that simple. Aluminum accumulation increases acetylcholinesterase activity which decreases acetylcholine levels. Aluminum accumulation often occurs in fungal and chronic bacterial infections. Most people with Lyme have fungal infections as well. Since aluminum is the 3rd most popular metal on earth and we Americans like to inject almost 5mg into our children in vaccines, many of us have aluminum accumulation issues. As the genetically modified plants produce more yield, they absorb more nutrients from the soil. This increases the aluminum content of the plants we eat and decreases their nutrient content. Some of us even breathe in aluminum these days and get it from the air. Antiperspirants, cookware, toothpaste, medications, many of these all contain aluminum. If I had anxiety, heart issues, or GERD, I'd be looking at aluminum as potentially being elevated.

What does DMSO do? It chelates aluminum and other metals.

Something to think about.

/www.earthclinic.com/cures/aluminum-poisoning.html
Posted 10/17/2017 12:41 PM (GMT 0)

Georgia Hunter said...
A great topic, but I wish it were that simple. Aluminum accumulation increases acetylcholinesterase activity which decreases acetylcholine levels. Aluminum accumulation often occurs in fungal and chronic bacterial infections. Most people with Lyme have fungal infections as well. Since aluminum is the 3rd most popular metal on earth and we Americans like to inject almost 5mg into our children in vaccines, many of us have aluminum accumulation issues. As the genetically modified plants produce more yield, they absorb more nutrients from the soil. This increases the aluminum content of the plants we eat and decreases their nutrient content. Some of us even breathe in aluminum these days and get it from the air. Antiperspirants, cookware, toothpaste, medications, many of these all contain aluminum. If I had anxiety, heart issues, or GERD, I'd be looking at aluminum as potentially being elevated.

What does DMSO do? It chelates aluminum and other metals.

Something to think about.

/www.earthclinic.com/cures/aluminum-poisoning.html

no idea if aluminum is a problem for lymies but if you are worried about it then try silicon water

https://www.ncbi.nlm.nih.gov/m/pubmed/22976072/

Somebody said...
Herein we are testing the hypothesis that silicon-rich mineral waters can be used as non-invasive methods to reduce the body burden of aluminum in individuals with Alzheimer's disease and a control group consisting of their carers and partners. We have shown that drinking up to 1 L of a silicon-rich mineral water each day for 12 weeks facilitated the removal of aluminum via the urine in both patient and control groups without any concomitant affect upon the urinary excretion of the essential metals, iron and copper. We have provided preliminary evidence that over 12 weeks of silicon-rich mineral water therapy the body burden of aluminum fell in individuals with Alzheimer's disease and, concomitantly, cognitive performance showed clinically relevant improvements in at least 3 out of 15 individuals.

Posted 10/17/2017 3:27 PM (GMT 0)
Lapis,

I can't thank you enough. Not because your recommended treatment will 100% work (it remains to be seen) but the fact that you do some good hardcore research and give people like me hope.

Choline:

"What is choline most beneficial for? Choline helps in the process of methylation, which is used to create DNA, for nerve signaling, and for detoxification. It’s also important for the functioning of a key neurotransmitter called acetylcholine, which similarly helps nerves to communicate and muscles to move, acts as an anti-aging neurotransmitter, and performs other basic processes."

This plus Huperzine A, theoretically and scientifically should work.

Of course the root cause, lyme and co, the pathogens causing this toxicity in the first place must also be dealt with BUT it finally gives us neuro-lymies some hope on managing this particular symptom as we continue to treat lyme.

Buhner whom has some very good scientific insight and help on many lyme symptoms lacks GREATLY in CNS, nerve, ALS-like symptoms.

You go to that section of his treatment recommendations and it's simply "eat shiitake mushrooms". Uhhh....no, that is NOT going to cut it.

With these particular symptoms it's not that the person isn't eating enough and eating enough protein, they are but it's almost in vain because the nerve signaling and communication necessary to not waste needs to improve first. THEN the protein and amino-acids needed to rebuild can be used.

It's like having all the pieces to build a house but no plot of land to put it on first. The house isn't going to get built.

So thank you Lapis, I am going to give this a try. Not sure if it will work when it's all said and done, but you giving me hard scientific evidence "hope" to try and not just the feeling of I'll treat lyme and cross my fingers. There may be something I can specifically do to manage this particular symptom.
Posted 10/17/2017 6:51 PM (GMT 0)
Yes thank you Lapis!

Question though. MY DMSO is arriving tomorrow. How do I apply. I have been reading up on it a bit. It sounds a little scary.

Use a cotton ball or something? Can't use dropper right because it will dissolve it right? How much to apply? Where to apply?

Will I smell super garlicy like some of the stuff I have been reading?

I will report back my experience.

Thanks again!
Posted 10/17/2017 8:12 PM (GMT 0)
@Lapis_29, thank you so much for this share! Very interesting.

On a related note, I found a video this week on YouTube that had *Contrary information* specifically about Acetylcholine pathways in the brain & lyme, amongst other factors. You can watch the video here:

https://www.youtube.com/watch?v=3xsQcThi4Pw

At first, I thought this guy was a bit of a wingnut, but he seems to have done quite a bit of research and seems to have a good grasp of the science. Then again, he *is* selling his proprietary herb blend, so I do take what he's saying with a grain of salt.

But none the less, this is an important video to watch before we all go taking supplements for acetylcholine, just in case it's not the right move. All the best to you all
Posted 10/17/2017 8:40 PM (GMT 0)

Dave Here Now said...
@Lapis_29, thank you so much for this share! Very interesting.

On a related note, I found a video this week on YouTube that had *Contrary information* specifically about Acetylcholine pathways in the brain & lyme, amongst other factors. You can watch the video here:

https://www.youtube.com/watch?v=3xsQcThi4Pw

At first, I thought this guy was a bit of a wingnut, but he seems to have done quite a bit of research and seems to have a good grasp of the science. Then again, he *is* selling his proprietary herb blend, so I do take what he's saying with a grain of salt.

But none the less, this is an important video to watch before we all go taking supplements for acetylcholine, just in case it's not the right move. All the best to you all

so glad you posted that video. prompted me to look deeper and check it out!

found a case study where a patient with severe neuro-lyme was cured with ABX and Pyridostigmine!

Pyridostigmine being the very drug I noted above that is in the family of Prescription drugs that increase acetylcholine, they are called Cholinesterase inhibitors

so honestly I think I am on the right path here.

Also that guy in the video has no sources, no studies, just "i read a thing somewhere once that said..."

well..what thing? where? when? can we read it?

and would it kill him to shave and throw on a shirt? lol! just kidding.

here is the case study.

https://www.ncbi.nlm.nih.gov/pubmed/17366045

Somebody said...


Scand J Infect Dis. 2007;39(2):187-90.


Myasthenia and neuroborreliosis with excessively high acetylcholine-receptor antibodies.


In a 29-y-old male with neuroborreliosis, partially responsive to ceftriaxone, myasthenia gravis with acetylcholine-receptor antibodies elevated almost 1000 times the upper reference limit was diagnosed. Pyridostigmine resolved all remaining neurological deficits. During a 1-y follow-up the patient remained symptom free, despite persistently high acetylcholine-receptor antibodies. They were attributed to epitope homology of the acetylcholine receptors and Borrelia surface antigens.

note that the acetylcholine receptor antibodies are extremely high. that means the body is starved for acetylcholine

Post Edited (Lapis_29) : 10/17/2017 2:44:35 PM (GMT-6)

Posted 10/17/2017 8:42 PM (GMT 0)
so a good thin g to do would be to get an acetylcholine receptor (AChR) antibody test

https://labtestsonline.org/understanding/analytes/achr-antibody/tab/test/

Somebody said...
AChR antibodies hinder the action of acetylcholine, a chemical (neurotransmitter) that transmits messages between nerve cells. The antibodies do this in three major ways:

"Binding" antibodies attach to the acetylcholine receptors on nerve cells and may initiate an inflammatory reaction that destroys them.
"Blocking" antibodies may sit on the receptors, preventing acetylcholine from binding.
"Modulating" antibodies may cross-link the receptors, causing them to be taken up into the muscle cell and removed from the neuromuscular junction.

Posted 10/17/2017 9:16 PM (GMT 0)
Still scared about DMSO, but I'll give the more "natural" Choline + huperzine A a try.
Posted 10/17/2017 9:16 PM (GMT 0)
Very Cool. Thank You so much for sharing all this, and for diving even deeper into the research. This is very hopeful news!

One reason I brought the video up is that during the onset of my symptoms last Fall, I had noticed that my response to a favorite Brain supplement (Onnit's Alpha Brain) had changed, along with my response to similar nootropics like Adrafinil & Piracetam (not sure if either of these work on the acetylcholine pathways or not).

Previously, I would feel great while taking Alpha Brain & afterward (which has Huperzine & Acetylcholine in it), but after the onset of my symptoms, I'd find that when it wore off, I was extremely depleted energetically, along with increased sensitivity to cold, muscle pains, and general weakness and mental slowness. I accepted some of that as just the 'cost of burning brightly' cognitively, but it was dramatically different from how Alpha Brain used to make me feel.

So the video caught my attention because I did indeed have a seeming correlation between this change in response and the onset of my symptoms.

Honestly, if this works, Between upping magnesium & Antioxidants, detoxing well, managing diet well and this new approach to acetylcholine, it seems like most of the symptoms I have will be covered. Exciting!
Posted 10/17/2017 9:23 PM (GMT 0)
Now it's about finding a high quality brand of Choline and Huperzine A.

Any suggestions?
Posted 10/17/2017 9:27 PM (GMT 0)
As part of our apitherapy tx i have ingested over a few kilos of royal jelly . This substance is very high in acetylcholine and other peptides .octopus grow back limbs with acetylcholine and im am noticing some of the wasting area coming back , Also as part of the pk protocol i have injected phosphtadylcholine and found sunflower lecithan to be a good nutritive source
/www.naturalnews.com/029069_royal_jelly_cognitive_function.html#
Posted 10/17/2017 9:47 PM (GMT 0)

bluelyme said...
As part of our apitherapy tx i have ingested over a few kilos of royal jelly . This substance is very high in acetylcholine and other peptides .octopus grow back limbs with acetylcholine and im am noticing some of the wasting area coming back , Also as part of the pk protocol i have injected phosphtadylcholine and found sunflower lecithan to be a good nutritive source
/www.naturalnews.com/029069_royal_jelly_cognitive_function.html#

intersting

how are you able to get that much royal jelly? are you buying it in bulk?
Posted 10/17/2017 9:53 PM (GMT 0)

dacarte3 said...
Now it's about finding a high quality brand of Choline and Huperzine A.

Any suggestions?

jarrow has a good choline supplement.

i have never used huperzineA
Posted 10/17/2017 10:25 PM (GMT 0)
Wait choline restores the health of already existing acetylcholine. It doesn't help produce more.

Is it a matter of needing more, or improving the health of the Neuro transmitter? Or both?

I'm just wondering if treating Lyme holistically, with all the anti-inflammatory herbs and supplements in these protocols including Pinella a CNS detoxing herb in addition to choline is enough or do I need huperzine A or DMSO to actually generate more?
Posted 10/17/2017 10:47 PM (GMT 0)
I keep reading more and more about choline. And this is the first thing that I've found that is DIRECTLY related to helping these symptoms (theoretically).

Not indirectly. Not do XYZ and it could maybe do this, that then does that, that then does this and in 6 months maybe it will help.

No. This is the direct, pin point, surgical, scientifically explained, thing I've been searching for. What causes these symptoms and why and then do XYZ to help reverse and repair the symptoms.
Posted 10/17/2017 11:34 PM (GMT 0)

dacarte3 said...
Wait choline restores the health of already existing acetylcholine. It doesn't help produce more.

Is it a matter of needing more, or improving the health of the Neuro transmitter? Or both?

I'm just wondering if treating Lyme holistically, with all the anti-inflammatory herbs and supplements in these protocols including Pinella a CNS detoxing herb in addition to choline is enough or do I need huperzine A or DMSO to actually generate more?

choline is the building block of acetylcholine

dmso/huperzine/Pyridostigmine are the stimulants to create more acetylcholine
Posted 10/17/2017 11:36 PM (GMT 0)
townsend news letter article which agrees with much of what I am saying in this thread

http://www.townsendletter.com/FebMar2006/lyme0206.htm
Posted 10/17/2017 11:42 PM (GMT 0)
ok, more great info!

turns out Huperzine A is a very weak stimulant of acetylcholine

however!

when you combine it with EGCG (green tea extract) huperzine is a very strong stimulant of acetylcholine


Somebody said...


Investigation of the Mechanism of Enhanced Effect of EGCG on Huperzine Aʼs Inhibition of Acetylcholinesterase Activity in Rats by a Multispectroscopic Method

Jianbo Xiao†‡, Xiaoqing Chen†, Lei Zhang§, Simon G. Talbot#, Gloria C. Li⊥ and Ming Xu*§⊥

Abstract

The mechanism of enhanced effect of (−)-epigallocatechin-3-gallate (EGCG) on huperzine Aʼs (HUP) inhibition of acetylcholinesterase (AChE) activity in rats was investigated.

The inhibitory effects of HUP at 10 and 5 µg/kg on AChE activity were quite weak in the whole phase. In contrast, upon addition of EGCG (100 mg/kg) to the HUP 10 and 5 µg/kg groups, remarkably enhanced inhibitory effects with maximum inhibitory percentages of 90.94 and 88.13% were observed under the same conditions. EGCG also can greatly prolong the inhibitory time.

The mechanism of the enhanced effects of EGCG on HUPʼs inhibition of AChE activity was investigated by steady fluorescence spectroscopy, infrared spectroscopy, and ultraviolet spectroscopy. HUP hardly interacted with the main transport protein, whereas there was a very strong binding interaction between EGCG and bovine serum albumin. The enhanced transport of HUP is a possible cause of the enhanced effect of EGCG on HUP bioactivity.



so basically if you are going to take Huperzine A, take some EGCG with it. Remember to include a choline supplement.
Posted 10/17/2017 11:49 PM (GMT 0)

Missouri said...
Yes thank you Lapis!

Question though. MY DMSO is arriving tomorrow. How do I apply. I have been reading up on it a bit. It sounds a little scary.

Use a cotton ball or something? Can't use dropper right because it will dissolve it right? How much to apply? Where to apply?

Will I smell super garlicy like some of the stuff I have been reading?

I will report back my experience.

Thanks again!

you can use nitrile gloves and just rub it on

put it anywhere and everywhere you hurt or have pain/inflammation

start wtih small amounts, work up to larger

make sure skin you apply it to is VERY CLEAN!!

I dont smell garlicy. Not sure why other do, wonder if they are using low grade dmso?

you might get a burning sensation, lasts 30 minutes or so

if skin gets dry afterwards, use vit E/aloe lotion

Post Edited (Lapis_29) : 10/17/2017 6:25:33 PM (GMT-6)

Posted 10/18/2017 1:14 AM (GMT 0)
I don't know if I'm allowed to post links to other commercial sites but google Alpha Brain. I've only heard good things about from Joe Rogan's podcast.

It has Huperzine in it:

"An extract from the plant commonly known as northern firmoss (Huperzia serrata), Huperzine A is a naturally derived acetylcholinesterase inhibitor. Acetylcholinesterase (AchE) is responsible for the breakdown of Acetylcholine, and so by inhibiting AchE, more acetylcholine is made available to the brain."

it even has cats claw in it haha, who knew.

240mg of Huperzine daily serving. I wonder if that is enough.
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