Posted 12/6/2019 10:22 PM (GMT 0)
ci Rep. 2015 Jul 30;5:12549. doi: 10.1038/srep12549.
Toll-like receptor 4 signaling in neurons of trigeminal ganglion contributes to nociception induced by acute pulpitis in rats.
Lin JJ1, Du Y2, Cai WK3, Kuang R4, Chang T5, Zhang Z5, Yang YX5, Sun C5, Li ZY5, Kuang F6.
Author information
1
1] Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710004, China Department of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, The Fourth Military Medical University, Xi'an, 710032, China.
2
1] State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032,China Department of Endodontics, Jinan Stomatological Hospital, Jinan, 250001, China.
3
1] Department of Cardio-Thoracic Surgery, Kunming General Hospital of Chengdu Military Region, Kunming, 650000, China Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710004, China.
4
State Key Laboratory of Military Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi'an, 710032,China.
5
Department of Neurology, Tangdu Hospital, The Fourth Military Medical University, Xi'an, 710004, China.
6
Department of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, The Fourth Military Medical University, Xi'an, 710032, China.
Abstract
Pain caused by acute pulpitis (AP) is a common symptom in clinical settings. However, its underlying mechanisms have largely remained unknown. Using AP model, we demonstrated that dental injury caused severe pulp inflammation with up-regulated serum IL-1β. Assessment from head-withdrawal reflex thresholds (HWTs) and open-field test demonstrated nociceptive response at 1 day post injury. A consistent up-regulation of Toll-like receptor 4 (TLR4) in the trigeminal ganglion (TG) ipsilateral to the injured pulp was found; and downstream signaling components of TLR4, including MyD88, TRIF and NF-κB, and cytokines such as TNF-α and IL-1β, were also increased. Retrograde labeling indicated that most TLR4 positve neuron in the TG innnervated the pulp and TLR4 immunoreactivity was mainly in the medium and small neurons. Double labeling showed that the TLR4 expressing neurons in the ipsilateral TG were TRPV1 and CGRP positive, but IB4 negative. Furthermore, blocking TLR4 by eritoran (TLR4 antagonist) in TGs of the AP model significantly down-regulated MyD88, TRIF, NF-κB, TNF-α and IL-1β production and behavior of nociceptive response. Our findings suggest that TLR4 signaling in TG cells, particularly the peptidergic TRPV1 neurons, plays a key role in AP-induced nociception, and indicate that TLR4 signaling could be a potential therapeutic target for orofacial pain.
I appreciate any help interpreting this.
Thank you