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Does anybody here have a background in biology?

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Posted 4/30/2017 10:29 PM (GMT 0)
Im looking for some help with an experimental idea that I have and the biology aspect of it is a little over my head.
Does anyone have at least a bachelors degree in bio or something similar?
Posted 5/1/2017 3:31 PM (GMT 0)
bump
Posted 5/1/2017 5:57 PM (GMT 0)
bump
Posted 5/1/2017 7:58 PM (GMT 0)
I have a B.Sc.
Posted 5/1/2017 8:06 PM (GMT 0)
I finish my bachelors in biochemistry in 3 days yeah
Posted 5/1/2017 8:25 PM (GMT 0)

Psilociraptor said...
I finish my bachelors in biochemistry in 3 days yeah

I knew you were a smart one😊
Posted 5/1/2017 8:50 PM (GMT 0)

Psilociraptor said...
I finish my bachelors in biochemistry in 3 days yeah

Congrats, Psilo!
Posted 5/1/2017 9:25 PM (GMT 0)
Lol i shouldn't be celebrating just yet. Didn't do so hot on my last inorganic chem test. But no worries OP i can discuss biology all day! Just don't look to me for questions coordination chemistry...
Posted 5/1/2017 9:33 PM (GMT 0)
I think marnie on lymenet does and georgia hunter has a working noggin.why do you ask maf? Tooty and lymed2010 and dude from k are the best microscopists around.
Posted 5/2/2017 2:02 AM (GMT 0)
Background in Biology and Chem.
Posted 5/2/2017 3:08 AM (GMT 0)

Somebody said...
Background in Biology and Chem.

Same here.

.......question is....can my Lyme brain remember any of it turn
Posted 5/2/2017 2:50 PM (GMT 0)
I learned the basics of microbiology from FUNDEMENTAL PRINCIPAS OF BACTERIOLOGY, also there is an on-line video course called SYNTHETIC BIOLOGY which goes into genetic engineering on a layman's level.

A project which I felt I would have to put a lab together and try something that would repair the DNA of the body.

NASA is planning on using this to allow their people to go to MARS as it will repair damage done by radiation and it also reverses "aging".

Another Todar ON-Line Biology book. It has a section on LYME's disease.

Present by using a red light I am able to increase the body's adenosine triphospate (atp) production which put energy directly into the cell. Able to increase wound healing by up to 200%.

But, I digress, the 2015 noble prize went to three researchers who discovered the chemical produced by the body to protect the DNA...

I have also removed oxidative stress using magnetic fields (it also kills cancer). I can remove between 60%-70% of free radicals (unpaired electrons or free radicals.

God Bless Claude
Posted 5/4/2017 3:18 PM (GMT 0)
I am in the process of getting a few things in order and then I will contact those of you who may be able to help. just hold tight for a few days and I will be back with more info. I have spoke to someone at Yale about the possibility of what I want to do and he agrees that it is possible AND applicable to lyme disease. More info soon. Thank you to those of you who have responded!
Posted 9/20/2017 4:36 AM (GMT 0)
Interesting. Let us know when you have an update.
Posted 9/20/2017 4:52 AM (GMT 0)
So, small update.... I tried what I set out to do.

The substance that I mentioned was a peptide(P1) that was made to mimic The Ixodes scapularis tick antifreeze glycoprotein, IAFGP. I ordered it from Genscript. It is not commercially available so I had the peptide sequence specially made. I got 14 grams for 200$

Here is one of the studies that I looked at to get my information

/www.ncbi.nlm.nih.gov/pubmed/28540585

I dissolved half of the peptide in a vial of pure water and did 0.1-0.7mg per day subcutaneous injections along with 100mg minocycline 2x per day and rifampicin 600mg once per day. All this for a total of 15 days.

I have saved the rest for my next round of antibiotics.
Posted 9/20/2017 8:12 AM (GMT 0)

MAF249 said...
So, small update.... I tried what I set out to do.

The substance that I mentioned was a peptide(P1) that was made to mimic The Ixodes scapularis tick antifreeze glycoprotein, IAFGP. I ordered it from Genscript. It is not commercially available so I had the peptide sequence specially made. I got 14 grams for 200$

Here is one of the studies that I looked at to get my information

/www.ncbi.nlm.nih.gov/pubmed/28540585

I dissolved half of the peptide in a vial of pure water and did 0.1-0.7mg per day subcutaneous injections along with 100mg minocycline 2x per day and rifampicin 600mg once per day. All this for a total of 15 days.

I have saved the rest for my next round of antibiotics.


You just have to be careful that your immune system doesn't react to the injected peptide. Not knowing the chemical structure of it, I can't comment on what it will do. Will a subcutaneous injection be sufficient to facilitate interaction with the EPS matrix of the biofilm?
Posted 9/21/2017 7:51 AM (GMT 0)

bobke said...
So the narrative that Biofilms are the reason your Lyme treatment is failing has gotten a lot of mileage and that narrative has been culturally accepted but other evidence has been relatively ignored.
A peptide is merely a primitive protein with less amino acids in the chain but a lot of things affect biofilms however the focus should be on something else.
buhnerhealinglyme.com/herbs/incanus-as-biofilm-buster/
"I am not a big fan of the biofilm hysteria that is common among the lyme community right now. ALL bacteria form biofilms. It is just a grouping of bacteria together in one location and the formation of a kind of rigid structure, similar to coral formation in the oceans, that they use to protect themselves. This is just ONE of a great many mechanisms bacteria use to protect themselves from immune responses or antibacterial substances and so on. It is no more dangerous or important than the bacterial ability to use efflux pumps to remove antibacterial substances from their cells or to use the immune system itself to hide from assault. Most herbal medicines are effective against biofilm formations just as are most immune systems. A biofilm may slow down effectiveness of immune response or herbal antibacterials but it does not stop them. It is not the terminator of bacterial protection. Biofilms have been around for eons and plants and immune systems have developed mechanisms for dealing with them. I would not worry about them as of being of particular importance in becoming healthy."
As Stephen Buhner has said all plants have defenses against biofilms. I have found that organic acids are particularly active in this way.
www.ncbi.nlm.nih.gov/pubmed/26411891
"Usnic acid inhibits biofilm formation and virulent morphological traits of Candida albicans"
It is the calcification that Bartonella uses for a defense mechanism that is the real problem.
www.ncbi.nlm.nih.gov/pubmed/17678436
“Bartonella-induced endothelial cell proliferation is mediated by release of calcium from intracellular stores.”
www.ncbi.nlm.nih.gov/pubmed/14759468
“Role of Bartonella henselae endocarditis in the nucleation of aortic valvular calcification.”
“Bacterial membranes contain calcium apatite crystals”
www.pnas.org/content/95/14/7846.full.pdf
“An infectious origin of extraskeletal calcification”
www.alive.com/health/the-nanobacteria-revolution/
"THE NANOBACTERIA REVOLUTION"
"Are heart disease, dental plaque, kidney stones, and a host of other calcium-deposit diseases really caused by an infection?"
What is missing from the current Lyme disease debate is context, people focus on one thing and run with it. You have to read an awful lot of science to realize that most of it is in error.

My newest bestist friend.
Posted 9/21/2017 8:57 AM (GMT 0)
Thanks for all the interesting and important information.
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