For those of you doing your own microscopy... *Part 3*

JohnB2 said...
Hello,

This may be the wrong forum for me. When squiggly lines and roundy things qualify as forms of borrelia for certain, but one can honestly watch 40 minutes of these videos just posted and not see any borrelia forms, I guess I don't know what to say. People are chasing after round dots that could be 500 different types of bacteria and calling them borrelia online. This is not a competition. We have family members that are sick.

johnB2 can you give an exact time stamp on one of your videos that show spirochetes forming a L-body ? thank you

We now have one of our Lyme microscopy checkers who has found a typical form of the spirochete in Lyme blood.
https://www.youtube.com/watch?v=4ZAyI9hODwg&feature=youtu.be

https://www.youtube.com/watch?v=FHCn2o7U8Lo&feature=youtu.be
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In this next video you will see also spirochetes wake up to drill just like that directly from ticks. The difference is that the one in ticks in this video is smaller & the one in the Lyme blood video is thicker.
https://www.youtube.com/watch?v=RnFR4Ca5MVo

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Here is another thicker one from a tick.
https://www.youtube.com/watch?v=-5UCWg-M3C4&t=10s
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Here they are fluorescent stained in mouse blood.
https://www.youtube.com/watch?v=THwcT1rjNXQ

Lapis_29 said...
john, I am truly not trying to debate you, I am sure you are doing great work, its just for some people an hour long video of microscopic objects is confusing thats all. I just dont know what I am looking at.

really no need to get so defensive. I hope you find a way to treat you and your family and everybody gets healed.

These are my thoughts as well. No offense was meant, was just having trouble seeing spirochetes in that video.

Yes, that is a spirochete that is drilling very fast in the plasma & around red blood cells of someone who is chronically ill for 40 years. Even in ticks some spiros can drill this fast.

John B, Acridine Orange can also stain indiscriminately & I had seen your AO stain in the past & those are not spirochetes. What happens is that the wbc's lyse & get strewn all over in the smear & you will get string-like subjects that are really part of the DNA from the Wbc's.For you to get a better idea, stain a normal persons blood & you will see the same artifacts. Full strength AO will be cytotoxic to Borrelia & can destroy most of them. Lab workers have also reported that full strength AO will also lyse sperm when they tried it & so they know it is toxic to certain cells in it's purer forms. Spirochetes are hardy yet delicate creatures.

I use live cell AO staining, in which I dilute AO .2% with NaCl (Salt water) & I give the exact dilution method that I used in the description of my video. Too concentrated & it can kill the spiros, not concentrated enough & one can miss the spiros if they are there. Sometimes even when you get the concentration right even the amount of AO drop that you use with the blood drop can alter the outcome, as it is hard to control the exact amount of a drop without a pipette.

When you look for AO stains in live preps, look for the known shapes, movements & morphologies (SOP or Tennis Rackets...etc) & not just any strings or string conglomerates. I hope this helps, as I want you to be successful too...all the best and if you need any help just LMK.

String of Pearls & 2 daughter splitting spiros in this video can be seen, as well as blebs/granular forms. Not all the green dots are spiros though, as some are from wbc components (lysosomes & peroxisomes).
https://www.youtube.com/watch?v=qlL8ZLitiBI