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Inhibition of Epstein-Barr Virus Lytic Cycle by an Ethyl Acetate from Polygonum cuspidatum

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Posted 1/14/2022 11:12 AM (GMT 0)
Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethyl acetate subfraction F3 obtained from P. cuspidatum root and its major component, emodin, for their capacity to inhibit the Epstein-Barr virus (EBV) lytic cycle. The cell viability was determined by the MTT [3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of EBV lytic proteins was analyzed by immunoblot, indirect immunofluorescence and flow cytometric assays. Real-time quantitative PCR was used to assess the EBV DNA replication and the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the F3 and its major component emodin inhibit the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduces EBV DNA replication, showing that F3 and emodin are potentially useful as an anti-EBV drug.

https://www.mdpi.com/1420-3049/19/1/1258/htm

So this is why Buhner prescribes knotweed, huh ...

and now with this new information: https://www.scientificamerican.com/article/epstein-barr-virus-found-to-trigger-multiple-sclerosis/

and the fact that knotweed is good against lyme too:
https://pubmed.ncbi.nlm.nih.gov/32154254/

...probably the best treatment for chronic lyme all is knotweed afterall. and i just thought this is just an adjunct treatment just to help detoxify/lower symptoms.
Posted 1/14/2022 12:11 PM (GMT 0)
I recall Buhner writing that the discovery of JK for Lyme was a real boon. My impression is that it is central to his regimen. I keep on seeing it mentioned for the coinfections and other things as well, and now this.

Also, Ivermectin is also getting some good EB press.
Posted 1/14/2022 3:57 PM (GMT 0)
I started Buhner’s protocols in 2017 and have continued taking knotweed ever since with or without other herbs. He said it was safe for long term use. I have only used powdered knotweed, so it’s very affordable. A 1 lb bag will last a few months. I keep it stored in a large mason jar kept in the hall closet. My impression from Buhner’s books was that knotweed was essential for both Lyme and bartonella and protects the endothelial cells.
Posted 1/14/2022 4:21 PM (GMT 0)
Buhner's approaches are all primarily designed to address infections by targeting the human cytokines that each infection causes to be dysregulated.

his theory is - that by reversing these inflammatory changes that the invading organism causes, you swing the needle away from favouring the pathogen and towards favouring human homeostasis and as a result the human immune system should be able to overcome the infection.

so in his mind - using his paradigm - knotweed does this for the main cytokines that are caused to be raised in Lyme - and is therefore primary by that definition
Posted 1/14/2022 4:23 PM (GMT 0)
You guys remind me, I might have to start Japanese Knotweed again, since I suppose I take a bulk supplement most often due to Buhners recommendations...(have 1 lb of Siberian Ginseng/Eleuthero as it a great adaptogen, I like to buy in bulk to save also WbF !)--I'm always leery of the dreaded Herx and get confused as to which herbs are massively bactericidal or more of a broad-spectrum combo-hit like adaptogen/modulation versus antibiotic, Cannabis is the most broad spectrum that way that I've found, if you find the right strain it can fight infection and calm the system at the same time which would be ideal...love the way Buhner talks about how an invasive species like JK starts growing wildly in Lyme endemic hotspots, like Mother Nature is already doing most of the guess work : )
Posted 1/14/2022 5:16 PM (GMT 0)
Protecting the endothelial cells is beneficial for more than just Lyme and bartonella.

“Pathogenic bacteria use the bloodstream as a highway for getting around the body, and thus have to find ways to enter and exit through the endothelium. Many bacteria approach this problem by producing toxins that can breach the endothelial barrier through diverse creative mechanisms, including directly killing endothelial cells (ECs), weakening the cytoskeleton within ECs, and breaking the junctions between ECs. Toxins can also modulate the immune response by influencing endothelial biology, and can modulate endothelial function by influencing the response of leukocytes. Understanding these interactions, in both the in vitro and in vivo contexts, is of critical importance for designing new therapies for sepsis and other severe bacterial diseases.” - from https://pubmed.ncbi.nlm.nih.gov/28013162/

My emphasis in bold. Preventing incoming pathogenic bacteria from getting around the body to make a home for themselves.
Posted 1/14/2022 5:20 PM (GMT 0)
Mpost,

Knotweed does cause herxes for some. It did for me, but nothing comparable to Sida acuta or houttuynia or cat’s claw. For me, my joint stiffness would increase when I would increase doses, but I persevered. I started with what was probably 1/16 - 1/8 tsp and just kept gradually increasing.

Houttuynia actually improved my joint stiffness at one time. I just restarted houtty last night. Hopefully, I will get some benefit out of it.
Posted 1/14/2022 9:20 PM (GMT 0)
yep for me Knotweed has always been pretty easily tolerated

i worked up initially with the tablets Buhner recommended ( Source naturals brand i think) 1tablet 4x a day for a week - the 2 for a week then 3 and then 4 - i don't recall having herx like reactions

I later took 20-30g per day of powder or 30ml tincture - and noticed no reaction in going from 20g to 30g ( 50% step )
whereas a 50% step in sida would give me severe reactions
Posted 1/14/2022 9:43 PM (GMT 0)

WalkingbyFaith said...
Mpost,

Knotweed does cause herxes for some. It did for me, but nothing comparable to Sida acuta or houttuynia or cat’s claw.

i know, i have not heard a lot of people saying "knotweed is powerful". yet after reading about inhibiting EBV, im thinking to give it more credit. There are a lot of options for lyme, but there are not so many options to inhibit Epstein Barr, you know... And seems EBV plays a major role in late MS like symptoms.
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