Somebody interested in interpreting a darkfield Video?

there is a lot going on in that slide Blazer

see markup snapshot here
VIEW IMAGE

as mentioned before in the other discussion - there is a huge amount of debris in the slide - way more than usual - and of course its all jiggling in the video in Brownian notion - so it looks alive / moving under its own steam - but all items including the thread like items are just moving in the Brownian motion - nothing is moving under its own volition here.

these thread like artefacts are actually quite common in blood samples - and occur more often in sick people - but studies have isolated and looked at them with PCR and found them to be human origin cellular debris -filamentous pieces of broken cell membrane - which fits with the huge amount of cellular debris in the plasma in the side.

people get a bit upset if i highlight this - as they are often convinced that these are spirochetes and are perhaps emotionally invested in that being the case - as some practitioners are also - there is not much i can do about that other than point to the evidence.

here is real video of actual live spirochetes - and you can see they are flat zigzag shaped things that move in a very characteristic jerky corkscrew movement
not lazy moving smooth curved wavy things just being passively jiggled as they are impacted by high energy water molecules - like everything in that video ( that is the cause of Brownian motion)

https://www.youtube.com/watch?v=cxyft5hsloq

there is also the size of these objects - its too small for real lyme disease spirochetes which are 10 to 30um long - a red blood cell is normally 8 or less if damaged - all of these threads are less than 1 redblood cell width across - ie around 7 or less - so outside the range for borrelial burgdorferi

just about all of the red blood cells are also damaged - crenelated cells or burr cells - highly unusual if the whole sample was like that - and again aligns with the expectation of and visual confirmation of lots of debris ( particles and threads) in the rest of the slide
by the way they will soon all be removed by the spleen if it is functioning properly - so i would expect some signs of at least mild anaemia to present ( again if all blood in the sample was like this)

its still possible the thread like objects are dead spirochetes - which could look a bit more like that - but this would be quite a big assumption - and not a safe one in my view

that's the think with microscopy - you can see shapes but cannot always properly identify them unless keep morphological features are present
its not as simple as all thread like things are spirochetes

again we know that actual lyme spirochetes are rarely found in blood of chronic patients even with PCR - which can detect a single spirochete in 0.5ml sample. so again - if its that uncommon in the blood that you have typically less than 1 spirochete per 0.5ml of blood - which is enough blood for around 20 slides - that it would take at least a week to look at all of those thoroughly and the chance of you spotting that one single spirochete in all of that time is minute - so that needle in a haystack is actually the normal citation for lyme in blood. not half a dozen in a single slide view (btw - 6 spirochetes in a slide corresponds to approx 400,000 more spirochetes than expected in a human blood sample)

so to recap
-they are too short - outside the range of BB
-they are not shaped like BB - smooth curves - not tight zigzag shapes along their length
-they are not moving like BB - smooth slow random waves - not BB's fast rhythmic jerky motion
-there are way too many of them

also logically - if it was so easy to simply put a drop of blood on a slide and look at it and diagnose Lyme - i think all the top LLMD's would just have a microscope in their offices - but they do not.

so i would say - keep confirmation bias in check - follow the multiple lines of logic to form an opinion

i am not saying you dont have lyme - just that people talk all sorts of unsupported nonsense about darkfield microscopy of whole blood, that simply doesn't add up if you dig into it

something is defo damaging your red blood cells though - so that needs looking into ( lyme itself is not really known for this ( of course bart or babs are as they actually invade and live inside RBC's )

that is what we in the microscopy group on lymenet flash call a "medusa" head . its an infected rbc with them "hatching" out . leave the smear under the light and rbcs will degrade on edges and stuff will come out . that looks very much like the borrelia darkfield that tlerks cultured in a bsk medium.

what video garzie showed was not borrelia but leptospirosis ; which has way different motility. here is a french video of borrelia , the european types are more virulent and neurologically manifested i hear .

https://youtu.be/0-XO-NSbsy0

the video i posted includes borrelia as well as Leptospira (that was just the first one in a series of spirochetes)

i thought it useful to see several examples of actual live spirochetes as they all have that jerky characteristic zigzag movement - in fact, multiple points of reference from related species are how morphological traits are often distinguished and refined in microbiology.

the many thread like objects in the French video are not very clear - could be almost anything - and again there is a huge amount of debris in the slide indicating breakdown of red blood cells - but what you can see is that they are again mostly around or less than a red blood cell across - ie 8um or less - so outside the range of borrelia burgdorferi

bluelyme said...
this aint reading teas leaves Garzie .its 1st year pathology 101. compare and contrast and do your own smears and stains and then one can say with some certainty. anything else is hearsay and misinformation. maybe persons have trouble with lightfield but with phase or darkfield there is no mistaking...

here is that bsk culture from the microscopy group , its clear as day

https://youtu.be/aFa6S8fB2Tk

https://youtu.be/dH3fGVndMYo

Thanks Blue

the first one is in my view clearly actual spirochetes moving across the slide under their own steam
these objects are the correct size for Borrelia Burgdorferi - being longer than a single blood cell is wide
- up to 3 or more time as as long as a red blood cell - its also normal to see this many organisms in a BSK culture - after a week or so - as that's what BSK is for
so - to summarise
-actual self propelled movement across the slide (not just drift or jiggling) = Check
-organisms length within the documented range of borrelia = Check
-numbers not out of line with expectation - as high numbers are normal in BSK culture = Check
so, everything here is consistent with what has already been discussed and these are to my mind almost undoubtedly spirochetes

however, and not wishing to offend, but anyone who cannot see the clear differences between the objects in this footage of a BSK culture - and those in the footage posted by blazer of fresh blood in the first post - hasn't done pathology 101!
sure they are both thread like objects - but again - to summarise
-self propelled movement across the slide - Nope - just jiggling on the spot (Brownian Motion)
-organisms within the known size range of borrelia burgdorferi - Nope - all below that range
-numbers in slide within expectation - nope - 1000's of times higher than numbers expected in fresh blood

in the end everyone has to make their own mind up - and its not my job to change it if someone is committed to one belief or another
i just want new members to be able to make their own mind up and not fall into the trap of confirmation bias or be abused by practitioners wanting to make a buck - or who are just somewhat ill informed themselves.

i am still ill after 8 years mainly because i have been told things that i believed to be true at the time -because the people telling me were apparently experts - but also because i was also so keen , if not desperate, as we all are, to find an explanation. this makes us very susceptible to suggestion.
But i later found much of what i was told by so called experts to be untrue - and each time this cost me months or years of my life going down some fruitless rabbit hole or other.

if there was anyone around to say at that time- "hang on, its not that cut and dried" -or - "there is more to the story" i would be much better off today - it could have saved me literally years of my life.

i am just trying to perform that function for others that i wish was around years ago.

again - i am not saying Blazer doesn't have Lyme - just that from the darkfield imagery posted
the thread like items do not match the documented morphology of borrelia in multiple ways
so, again - in my view, based on the points I raised, it does nothing to confirm or refute a Lyme infection