Yellow Cat said...
I know there is company close to my place which makes live blood analysis with microscope of 1600x zoom, is it enough to see spirochetes?
1600x is way more than you even need. Darkfield (which is usually what live blood analysis consultants are using) at 400x is sufficient to see spirochetes.
Just be aware that not all live blood analysists are doing the scanning properly. Many scan the blood immediately after drawing, but that is not always enough time for the spirochetes to leave the cells to be visible in the plasma. We sometimes have to leave the sample sit under the microscope for 12-48 hours. I initially had a analysist look at my blood and he looked for maybe 3 minutes. Not surprisingly, he saw no spirochetes. So, just because they don't see any spirochetes in your sample during your visit, if left under the scope longer, they may be visible. Also, some analysists may not be interested in finding spirochetes, but only cell abnormalities which they can supposedly address with their proprietary herbs and supplements. That way they minimize liability because they are not diagnosing diseases or infections, only "nutritional deficiencies." The way they go about
finding nutritional deficiencies on a live blood smear is usually hocus pocus. The condition & appearance of the cells will vary depending on the
location in the sample. Cells at the edge of the smear look abnormal and this area usually has lots of rouleaux. Cells at the center of the sample usually look round, evenly spaced, and normal. The appearance also depends a lot on the amount of blood under the coverslip. Excess blood=more rouleaux. Less blood= less rouleaux and evenly spaced cells. So, don't be fooled by that bullcrap. The main things to look for in live blood samples are the presence of spirochetes, the health and motility of white blood cells, and you can perhaps get a general idea of a white blood cell differential. One other thing I have found telling are what I call "garbage dumps." You'll know what I am referring to when you see them. I noticed that I had more of them when I was sicker. Some people think they are clumps of candida organisms, but I have decided they are unspecified clumps of pleomorphic L-form organisms. Who knows what they are, but if you see them, I think it means you are one sick kiddo!
So, live blood is not useful beyond these things, unless you have fluorescent stain such as acridine orange. The main thing it's useful for then is verifying a borrelia infection. For that, it's worth A LOT!
The best way is to buy a microscope yourself (preferably darkfield or phase contrast) and look at your blood yourself. Consider that the sick have the time and incentive to really explore their blood in depth. No doctor, analysist, or pathologist is going to spend that much time on your particular sample. That said, a good live blood analysist that has years of experience is going to be a good place to start if you do not have the wherewithal to look yourself.
Even the CDC recognizes that live blood microscopy is the best way to diagnose a Borrelia infection:
/www.cdc.gov/relapsing-fever/clinicians/index.html"Laboratory Testing
Spirochetemia (spirochetes in blood) in TBRF often reaches high concentrations, 100-1000 times more than in cases of early Lyme disease. Thus, microscopy is a useful diagnostic tool for TBRF. The definitive diagnosis of TBRF may be based on the observation of relapsing fever spirochetes (Borrelia hermsii, B. turicatae, or B. parkerii in the US) in peripheral blood smears of a symptomatic person by a microscopist trained in spirochete identification. Although best visualized by dark field microscopy, the organisms can also be detected using Wright-Giemsa or acridine orange stains.
....The organisms are best detected in blood obtained while a person is febrile. With subsequent febrile episodes, the number of circulating spirochetes decreases, making it harder to detect spirochetes on a peripheral blood smear. Even during the initial episode spirochetes will only be seen 70% of the time."
Notice that the main differences between Relapsing Fever spirochetes and Lyme disease spirochetes as it pertains to microscopy is that the load in early infection is 100-1000x greater for Relapsing Fever, and that Relapsing Fever spirochetes stain well with Giemsa. Borrelia burgdorferi spirochetes rarely, if ever, stain with Giemsa.
Post Edited (TOOTY) : 7/11/2017 2:20:45 PM (GMT-6)