Question about dna connexions results

Hi, wanted to know if anyone has had similar results on there DNA connexions results and any ideas what this means?

https://gyazo.com/6cf7ded21deb3eeb4129708c24e653c1


I'm extremely confused by the -IND at the end, seems like others on this forum all tend to have -NPS and I've seen some with neither. This still indicates a positive PCR right without a doubt?

DNA Connexions included this explanation with my results several years ago:

DNAConnexions said...

Interpretation of Results

Positive
A Positive result is indicated by the organism or gene being highlighted in yellow.

When our species-specific primer sets are designed, the expected size of the amplification product (in base pairs)
is known. As every patient sample, every positive control, and every negative control are run in duplicate, when a sample
produces an amplification product of the expected size on either, or both, of the assay runs, it is scored as a ‘positive’.

Non-Predicted Size (NPS)
A NPS result is indicated by the organism or gene being highlighted in yellow, followed by NPS.

When we see an aberrantly sized amplification product, we consider the following: These microbial genomes are
small, on the order of a million bp, and tend to reproduce quickly. If a mutation occurs within the genome, something
non-deleterious to the organism, the mutation will then be perpetuated in subsequent generations. If this mutation occurs
in our target amplification region, the size of the subsequent amplification product will change (small or larger). The
product size differential could possibly be due to mutation, degraded DNA, mutation of species, unspecified subspecies,
etc. We have found that the NPS are more commonly detected in individuals with long-term infections. To be scored as
an NPS, the product needs to be visualized on both runs of the sample. It is at the discretion of a qualified medical
provider to interpret NPS results.

Indeterminate (IND)
An Indeterminate result is indicated by the organism or gene being highlighted in yellow, followed by IND.

An Indeterminant result indicates that an amplification product was produced for a particular organism on the
panel. However, the amplification product was not the expected size and was only present on one test run of the sample.
The explanation for this is the same as the NPS, however the aberrant amplification product was only present on one test
run. It is at the discretion of a qualified medical provider to interpret IND results.

I also found DNA connexions to be inaccurate

Although some find it useful…

I found DNA ConneXions very useful, personally.

LabCorp tests only picked up Borrelia hermsii for me. IGeneX was indeterminate.

Antibody tests weren't picking up much for me because my immune system was so disabled and I wasn't making antibodies. A PCR test like DNA ConneXions seemed like a better alternative, and sure enough it did show the three Bs for me.

Interestingly, the species of Bartonella it showed (bacilliformis) has distinctive red wart-like growths that I had soon after being infected, so I feel confident that it was correct.

Once I was feeling better and my immune system was more functional, I started testing positive for Borrelia bergdorferi on regular LabCorp antibody tests.

If someone asks, I still say that IGeneX is the way to go if someone is only going to get one test, but I'm glad I had the DNA ConneXions test.

Girlie said...
Welcome to the forum, Johnny.
The “Ind” means indeterminate - something “showed up “ but not enough to be called a positive.

Thank you so much for the reply, that is interesting and I kind of thought that is what it meant. But its still extremely confusing, I am new to all of this but here is my situation;

The DNA connexions results all indicated indeterminate, I had another lymes test from another company which showed all negative PCR, for Borrelia, Bart, Babesia, and then a few slightly elevated IgG for Borrelia, but all the IgM were completely normal. Again, not sure how to interpret this when I consider both tests. Symptomatically sure it matches Lymes to some extent, a few symptoms here and there but I also am missing many of the key indicators, or at least the ones I think of. For example with Babesia infection, I have no Anemia, never had air hunger, or any of the other symptoms like that. Fatigue, joint pain, muscle twitches, POTS, ext I have, but I guess I don't know if any other chronic infection could cause these. To add on, I just looked at DNA connexions website and they say in chronic infections they typically state "NPS" in most cases, whatever is going on has been happening for well over 4 years at this point.

Would you say without a doubt Igenex is the best testing? Is there ANY testing I can do to be 100% certain that this is what I'm dealing with? I'm just so uneasy at this entire situation, especially when I have inconclusive results on 1 test, and another showing "nothing" essentially besides some elevated IgG levels, or maybe that is enough to determine I for sure have Borrelia? I don't know.

I had a positive result for Lyme and Ehrlichia from DNA Connexions. I had the NPS annotation on my results, which fit my scenario as I had been sick for many years. This was the only positive test I got. My Igenex test was negative. (I was positive on band 41 and indeterminate on 39.)

Interestingly, no coinfections other than ehrlichia were positive on my DNA Connexions test, even though I had bart marks since childhood and had many symptoms of bartonella and babesia in childhood according to some of the symptom lists I saw.

I was tested in early 2017. Their tests may have changed some since then.