Posted 1/13/2024 6:32 PM (GMT 0)
well done!
just some basic reagents / lab supplies
- giemsa stain powder* - suppliers on ebay
- immersion oil ( some should come in the kit with the scope ) - if not 5ml of thick type synthetic is best
- lab grade 99.99% methanol to dissolve stain in - and for fixing slides prior to staining - ebay labs supplies
- some clean pipettes for drip staining - disposable are fine - ebay or AliExpress are the least expensive
- some clean glass jars for rinsing slides in after staining - i used jam jars thoroughly cleaned
- filter paper for straining stain - if mixing own
- glass or plastic funnel to hold filter paper for filtering stain
- a Koplin jar - its much better for staining via immersion vs dripping - but you can start with dripping - i just
found it causes way more artefacts ( and these are in general the bane of microscopy work on blood )
- the guides say to use pH Buffered distilled water for rinsing and developing the colours in the Gimesa - but its not typically far from the range most tap water will be in - so you may be able to get away with that - i did
*i have not found a good place to buy ready mixed stain - so i have been mixing my own - which is a bit of a PITA - i recall DCD found a supplier of ready mixed giemsa type stain in the USA
suppliers of stain powder on ebay -
you will need glass beads to aid the mixing process
i think the link to the WHO guides on mixing the stain and using it may be in that post i mentioned - if not let me know and i will find them
top tips
1, be sure to go to town on cleaning the slides well with washing up liquid and water and then again with methanol prior to doing smears - as otherwise the blood will "fish eye" with any trace of grease
2a, buy some slides with ground edges - you need at least one of these for doing the smears - as rough as-cut edges will not smear evenly enough to be useful.
2b, learn the technique for doing a thin blood smear - practice it many times before getting acceptable smears to stain - see youtube for instructional vids - again i think there is a link in the thread i linked
3, cleanliness / avoiding any contamination is important as every spec of dust, fibre or contaminant can look like bacteria or parasites and lead you astray - so you need to be certain what is on your slide came from you blood - not the reagents or the environment
its a learning curve - but a rewarding one...
good luck!